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Research is exciting because it is about finding solutions, understanding failure, asking the right question, coming up with the wildest hypothesis, and thinking outside of the box.
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My scientific interests are diverse, however, throughout this journey, I have discovered that I am mainly drawn to engineering, technology development and project management.
In February 2023, I joined the lab of Prof. Dr. Randall Platt as a PhD candidate
Favourite subjects
The ability to design and engineer DNA in order to gain a deeper understanding of mechanisms and to advance medicine and manufacturing. I specialized in the CRISPR/Cas9 Prime editing technology.
The capacity to drive progress and innovation across various scientific fields by utilizing novel tools and methodologies to enhance research. My PhD project focuses on bridging two cutting-edge technologies.
The skill to lead a team towards its objectives, considering factors such as constraints, people, time, budget, and more. Throughout all the projects I have been involved in, one of my most valuable abilities has been my organizational and social skills.
Background: Gene expression is generally regulated by a complex network of regulatory elements. Objective: Understand the interaction and role of each of these regulatory elements. Method: Insertion of recombinase sites (LoxP and LoxPsym) by CRISPR/Cas9 Prime Editing between target gene regulatory elements in human cell line models. Induction of random rearrangement of the regulatory region. Sequencing of the new regulatory region architectures to match different expression levels of the target gene.
Background: Study of proteins involved in post-transcriptional processes and translation in plant mitochondria. Objective: Development of tools to study the interaction between two mitochondrial proteins in plants. Method: Cloning and insertion of the CRISPR/Cas9 Prime Editing technology in A. thaliana via a binary vector.
Background: Non-volatile compounds cannot be analysed by GC-MS. A method for derivatization of these compounds is therefore necessary. Objective: Analysis of secondary metabolic compounds from beer, human serum and sugars in GC-MS and LC-MS. Method: Derivation of non-volatile compounds by silylation (TMS) of beer, human serum and sugars for GC-MS analysis. And comparison with the LC-MS method.
Optimizing production of nanobodies in Pichia pastoris (yeast) in baffled flasks and then in a 5L bioreactor tank. Working in a clean room with GMP (Class C). Realization of a cross-flow filtration (TFF).
Missions: Helping 1st year student to understand biology concepts and guiding them towards an autonomy for college. Maintaining a regular monitoring of the students during the semester. Managing a small team of tutors.
Tri-national engineering school in biotechnology (English, German, French). Lectures on: Enzymology, Thermodynamics, Structural biology, Chemistry, Plant Physiology,Neurobiology, Genetic engineering, Immunology, Bioethics, Finances, Quality,Statistics, Molecular modelling, Process engineering.
Ecotoxicology, Bioremediation, plant bio-engineering, plants protection,interactions between plants and microbes.
Genetics, Biochemistry, Cellular biology, Immunology (in plants and animals), Microbiology, Cellular signalisation, Biostatistics,Populations et ecosystems.
M. Gouley, J. Koeppel, L. Parts (2022). “Randomizing gene regulatory regions using CRISPR/Cas9 Prime editing and the Cre Lox system”. Poster Session.
Download the posterMolecular Biology
PCR
Agarose gel
SDS-PAGE
Western Blot
Biosafety level 2
Genetic engineering
CRISPR/Cas9 Prime editing
Creation of stable cell line
Golden Gate cloning
DNA extraction
Cell culture
K562, HAP1, HeLa, iPSCs
Transfection, Infection, Electroporation
Immunostaining, FACS
Neural directed differentiation
Bioproduction
Pichia pastis culture
Production in a Bioreactor 3L and in baffled flasks
Cross-flow filtration
GMP Class C
Sequencing
Sanger Sequencing analysis
Cas9 directed Nanopore sequencing
Bioinformatic analysis of sequencing
Microbiology
E.coli and Agrobacterium
Media preparation
Mini and MidiPrep
Bacterial identification
Biosafety Level 2
Chromatography
GC-MS, LC-MS
Ion exchanger (AKTA)
Gel filtration
"Mélanie revealed herself to be highly motivated and focused, demonstrating an open and very attentive mind. With a great enthousiasm, she invested a lot of energy in every aspect of her group's project."
"During her internship, I was able to appreciate her cleverness, her technical skills, her initiative and her great reliability as well as her good interpersonal contact."
"The defining characteristic of Mélanie’s stay with us has been her highly proactive approach to her science and project choices. First, she started to suggest her own, often creative, and excellent ideas for novel experiments as early as the second week of her placement."